- Product Details
Keywords
- Heparinase
- Reagent
- diagnostic reagents
Quick Details
- ProName: Heparinase II, from Flavobacterium Hep...
- CasNo: 149371-12-0
- Application: Heparinase II is useful in many applic...
- ProductionCapacity: Metric Ton/Day
- Purity: ≥ 98 % by reversed phase HPLC analysi...
- LimitNum: 0 Metric Ton
Superiority
Heparinase II cleaves, via an elimination mechanism, sulfated polysaccharides chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate acvitity being about twice as high as the heparin activity.
Details
Properties
Molecular weight: 84.1 kDa
Isoelectric point: 9.1 – 9.2
pH optimum for activity: 7.6
pH range for activity: 5 – 9
Optimal temperature range: 20 ℃ – 37 ℃
Purity: Greater than/equal to 98% by reversed phase HPLC analysis
Protein impurities: < 0.1%
Specific Activity: > 15 IU/mg (heparin as substrate), >18 IU/mg (heparan sulfate as substrate)
Concentration: 4 IU/mL
Standard: 0.1 IU, 1.0 IU, or customized standard.
Unit Definition: One international unit (IU) of heparinase II is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 35 ℃ and pH 7.0.
Stability: Expiration of heparinase II is 12 months from manufacturing date frozen at – 20 ℃ in PBS.