China (Mainland)
Chondroitinase B CAS NO.52227-83-5
- Min.Order: 1 Metric Ton
- Payment Terms: T/T
- Product Details
Keywords
- Chondroitinase ABC
- Reagent
- diagnostic reagents
Quick Details
- ProName: Chondroitinase B
- CasNo: 52227-83-5
- Application: Determination of contents of chondroit...
- DeliveryTime: two weeks
- PackAge: stored in vials
- Port: Beijing
- ProductionCapacity: 1000 Metric Ton/Day
- Purity: ≥ 90 % by reversed phase HPLC analysi...
- Transportation: AIR shipping
- LimitNum: 1 Metric Ton
Superiority
Properties:
Molecular weight: 55kDa
Isoelectric point: 9.4 – 9.6
pH optimum for activity: 7 – 8
pH range for activity: 5 – 10
Optimal temperature range: 20 ℃ – 37 ℃
Purity: ≥ 90% by reversed phase HPLC analysis.
Standard: 1 IU and 5 IU.
Specific Activity: Greater than/equal to 200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amout of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.
Stability: 12 months froze at– 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.
Details
Chondroitinase ABC cleaves, via and elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopty at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.
Substrate Specificity: Chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.