China (Mainland)
Heparinase III, from Flavobacterium Heparinum CAS NO.37290-86-1
- Min.Order: 0 Metric Ton
- Payment Terms: T/T
- Product Details
Keywords
- Heparinase
- Reagent
- diagnostic reagents
Quick Details
- ProName: Heparinase III, from Flavobacterium He...
- CasNo: 37290-86-1
- Application: Heparinase III is useful in many appli...
- ProductionCapacity: Metric Ton/Day
- Purity: ≥ 98 % by reversed phase HPLC analysi...
- LimitNum: 0 Metric Ton
Superiority
Heparinase III cleaves selectively, via and elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active only towards heparan sulfate and does not cleave heparin or low molecular weight heparins.
Details
Properties
Molecular Weight: 70.8 kDa
Isoelectric point: 9.6 – 9.9
pH optimum for activity: 7.3
pH range for activity: 4 – 9
Optimal temperature range: 20 ℃ – 37 ℃
Purity: Greater than/equal to 98% by reversed phase HPLC analysis
Protein Impurities: < 0.1%
Specific Activity: > 200 IU/mg (max. 235 IU/mg)
Concentration: 5 IU/mL
Standard: 0.1 IU, 1.0 IU, or customized standard.
Unit Definition: One international unit (IU) of heparinase III is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from heparan sulfate per minute at 35 ℃ and pH 7.0.
Stability: Expiration of heparinase III is 12 months from manufacturing date frozen at – 20 ℃ in PBS.