- Product Details
Keywords
- Heparinase
- Reagent
- diagnostic reagents
Quick Details
- ProName: Heparinase I, from Flavobacterium Hepa...
- CasNo: 9025-39-2
- Application: Heparinase I is useful in many applica...
- DeliveryTime: two weeks
- ProductionCapacity: Metric Ton/Day
- Purity: ≥ 98 % by reversed phase HPLC analysi...
- LimitNum: 0 Metric Ton
Superiority
Heparinase I cleaves selectively, via and elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithroombin III binding pentasaccharide domain in the heparin molecule.
Details
Properties
Molecular weight: 42.8 kDa |
Isoelectric point: 9.3 – 9.5
pH optimum for activity: 7.4
pH range for activity: 4 – 9
Optimal temperature range: 20 ℃ – 37 ℃
Purity: ≥ 98 % by reversed phase HPLC analysis
Protein impurities: < 0.1%
Specific Activity: > 400 IU/mg (max. 417 IU/mg)
Concentration: 10 IU/mL
Standard: 0.1 IU, 1.0 IU, or customized standard
Unit Definition: One international unit (IU) of heparinase I is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 35 ℃ and pH 7.0.
Stability: Expiration of heparinase I is 12 months from manufacturing date, frozen at -20 ℃ in PBS.